데이터 자료를 살펴보세요

Figure 1. Carbamazepine Block of action potential firing. Figure 1 plots the number of evoked action potentials at the pacing frequencies of 0.1Hz, 1Hz, 3Hz and 10Hz in 10 neurons that were exposed to two sodium channel antagonists, carbamazepine and PF-05089771. Error bars are SEM. At 0.1Hz, the neurons are stimulated by a train of 10 current pulses. At 1Hz, 3Hz and 10Hz, the neurons are stimulated by a train of 120 current pulses at the respective frequencies. Both Carbamazepine and PF-05089771 inhibited action potentials in a concentration and frequency-dependent manner. 

Figure 2. Inhibition of TTX-R Currents in Human Dorsal Root Ganglion Neurons by Known Nav1.8 Blockers. In Figure 2 (above), human donor DRGs were procured using AnaBios’ proprietary surgical techniques and shipped in a proprietary neuroplegic solution to AnaBios via dedicated couriers. The DRGs were then dissected in cold neuroplegic solution to remove all connective tissue and fat. The ganglia were enzymatically digested, and the isolated neurons put in culture.

Cells were plated on coated PDL-coated coverslips and recordings were made over the first 8 days-in-vitro. Currents were recorded with an Axon Instruments 700A amplifier and Clampex acquisition software. Signals were filtered at 3 kHz and sampled at 10 kHz. Currents were leak-subtracted using a P/-4 protocol. Sodium currents were elicited with a 50 ms voltage pulse to the peak of the I-V curve from a holding potential of -80mV. Inter-stimulus interval was 10 seconds.

Internal recording solution contained (in mM): CsF (135), MgCl2 (1), Na-ATP (2), EGTA (1), HEPES (10). External recording solution contained (in mM): NaCl (20), Choline-Cl (95), KCl (3), MgCl2 (1), CaCl2 (2), CdCl2 (0.1), NiCl2 (0.1), glucose (25), HEPES (10), TEA (20), TTX (0.0005). Both test agents were dissolved in DMSO (final DMSO concentration 0.1%).

Figure 3. Schematic of Calcium Transients Recording in Human Dorsal Root Ganglia neuron. In Figure 3 (above) is a schematic figure of the recording of calcium transients (calcium steps) in a human DRG neuron loaded with Fluo-8 and stimulated by electrical field stimulation. The neural excitability in the form of evoked calcium steps can be measured simultaneously. The effect of a compound inhibitor can be measured by comparing the number of calcium steps pre and post addition of the inhibitor A. 

Figure 4. activation of calcium transients