l단일농도 혹은 IC50과 Ki
(binding affinity) 확인을 위한 Dose-response 실험
lMembrane preparation 직접 제작
lMajority of membrane preps produced
in-house
lRadiolabeled antagonist (Agonist
Radioligand Binding Assay) 혹은 agonist (Agonist Radioligand Binding Assay) 사용
이런 서비스 유형을 제공합니다
서비스 유형
GPCR Radioligand
Binding Assays
Item no.
타겟 선정
Target 수
선택 가능 (약 150개 타겟에 대한 260여개 Assay)
실험농도
선택 가능
농도 개수
단일 농도 혹은 8개 (Dose-response)
반복 실험 횟수
Duplicate
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Pharmacology of several adrenergic compounds at the alpha1A adrenergic receptor. The alpha1 antagonists WB 4101 and
prazosin, alpha1 agonist cirazoline and endogenous ligand epinephrine were
tested in a binding assay using [3H]prazosin (0.1 nM) as a radioligand and
membranes obtained from CHO cells transfected with the human alpha1A receptor.
WB 4101 is used as a standard reference compound for the assay and tested in
each experiment with client compounds. Data are expressed as % of control
specific [3H]prazosin binding.